FIG 4.
In vivo infections using Tn mutants with a transcervical mouse infection model. Groups of 10 female C57BL/6 mice were infected transcervically with 5 × 105 IFU of either parental (WT) C. trachomatis or Tn mutants as indicated. Five days postinfection, genital tracts were harvested and DNA was purified from uterine horns. Bacterial burdens were calculated as levels of Chlamydia genomes relative to host genomes, and ratios are shown as box-and-whisker scatter plots representing data from 10 mice. *, P < 0.05 (unpaired Student's t test).