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. 2019 Aug 7;9:11457. doi: 10.1038/s41598-019-47927-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The ASNase-carried PC9 or A549 stable cells depleted the asparagine in the medium and caused the cytotoxicity to the co-cultured parental PC9 or A549 cells. (a) The experimental design of the transwell co-culture assay. The doxycycline-inducible ASNase-carried PC9 or A549 stable cells were seeded in the inner chamber, and the parental PC9 or A549 cells were seeded in the outer chamber. After 48 hours of the asparaginase induction by doxycycline, the cell viability was assayed for the parental cells in the outer chamber. (b) The cell viability was compared between the doxycycline-treated and the non-treated experiments for PC9 in RPMI or DMEM medium. (c) same as (b) for A549.