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. 2019 Mar 8;17(9):1814–1822. doi: 10.1111/pbi.13102

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© 2019 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Efficiency of PVY^O inhibition correlates with Cas13a/sgRNA expression levels. (a) Disease symptoms in response to PVY^O infection in two transgenic S. tuberosum lines with high (St‐CI#5) and low (St‐CI#7) levels of Cas13a/sgRNA at 25 dpi. Virus accumulation was assessed at 25 dpi by enzyme‐linked immunosorbent assays (b) and quantitative reverse transcriptase‐polymerase chain reaction (c). Error bars represent SE. Data represent three biological replicates. Statistically significant differences are indicated by different letters (P < 0.05). (d) Northern blot analysis confirms the correlation between Cas13a/sgRNA expression level and the strength of the interference with PVY^O interference. RNA extracted from systemic leaves of infected plants was probed with a DIG‐labelled Potato virus Y (PVY) complementary RNA fragment (of 480 nt) targeted to coat protein and detected with an anti‐DIG antibody. The arrow indicates the full‐length PVY genome. The ethidium bromide‐stained rRNAs in the agarose gel prior to blotting is shown below the blot as loading control.