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. 2019 Mar 8;17(9):1814–1822. doi: 10.1111/pbi.13102

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© 2019 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Transgenic potato plants display specific resistance to Potato virus Y (PVY). PVY^N and PVY^N ^:O accumulation in transgenic Solanum tuberosum plants was assessed 25 dpi by enzyme‐linked immunosorbent assays (ELISA) (a and c) and quantitative reverse transcriptase‐polymerase chain reaction assays (b and d). Potato virus S (PVS) (e) and Potato virus A (PVA) (f) accumulation in transgenic S. tuberosum lines was assessed at 25 dpi by ELISA. Error bars represent SE. Data represent three biological replicates. Asterisks indicate statistically significant differences (*P < 0.05, independent‐samples t‐test). (g) Alignment of the CP‐sgRNA‐binding sequence from PVY^O with the corresponding regions in the coat protein (CP)‐encoding genes of PVA and PVS. The CP‐sgRNA designed to target PVY shows low sequence similarity to the CP region of PVA and PVS. Shading denotes nucleotides conserved in two viruses, asterisks mark nucleotides conserved in all three viruses.