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. 2019 Mar 5;17(9):1788–1803. doi: 10.1111/pbi.13100

Figure 6.

Figure 6

Functional analysis of SiSAM, a drought tolerance candidate gene in Sesamum indicum. (a) Various serial dilutions of yeast transformants carrying the candidate gene SiSAM C could grow normally in a MS medium supplemented with 2.5 m Mannitol in contrast to the yeast transformed with the control vector PYES2. (b) Phenotypes of wild type (WT) and transgenic Arabidopsis thaliana plants (L1‐L3) over‐expressing SiSAM C , under 150 mm Mannitol after 7 days. (c–d) Phenotypes of wild type (WT) and transgenic Arabidopsis thaliana plants (L1) over‐expressing SiSAM C , after 17 days water stress (S) and normal (CK) conditions. (e) RT‐PCR analysis of transcript levels of SiSAM C in the three transgenic lines and wild type (WT) plants. (f) Relative root length (RL), survival rate (SR) and relative silique number (SN) of the three transgenic lines and wild type (WT) plants. (g) Malonaldehyde (MDA) content of the three transgenic lines and wild type (WT) plants after 17 days water stress (S) and normal conditions (CK); (H‐K) Total adenosyl methionine (SAM), putresine (Put), spermidine (Spd) and spermine (Spm) in wild type (WT) plants and transgenic Arabidopsis thaliana lines (L1, L2 and L3) over‐expressing SiSAM C after 17 days water stress (S) and normal (CK) conditions. The average data and standard errors were calculated from three independent experiments. Bars with asterisks indicate lines that are significantly different to the wild type plants (***P < 0.001).