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. Author manuscript; available in PMC: 2019 Aug 8.
Published in final edited form as: Bioconjug Chem. 2018 Feb 19;29(4):1209–1218. doi: 10.1021/acs.bioconjchem.8b00004

Figure 2.

Figure 2.

Characterization of FTL-5X and FTL-5X/anti-ICAM conjugates. (a) Western blot (staining for hFTL), (b) fluorescence scan, and (c) SDS-PAGE analysis of FTL-5X conjugated to AlexaFluor488 labeled anti-ICAM at different molar ratios. Lane numbers indicate the following: (1) FTL-5X, (2) anti-ICAM, (3) FTL-5X/anti-ICAM at molar ratio of 1 to 2, and (4) FTL-5X/anti-ICAM at molar ratio of 1 to 10. TEM images and size analysis of FTL-5X and antibody conjugates. TEM images of (e) FTL-5X and (f) FTL-5X/anti-ICAM. Scale bar: 100 nm. Particle size analysis (size distribution by number) obtained by DLS for (d) FTL-5X, and FTL-5X/anti-ICAM, as well as their (g) mean size and polydispersity index (PDI).