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. Author manuscript; available in PMC: 2019 Aug 8.
Published in final edited form as: Science. 2014 May 9;344(6184):641–645. doi: 10.1126/science.1251102

Fig. 3. Functional phenotype and persistence of ERBB2IP mutation–specific CD4+ T cells.

Fig. 3.

(A) Patient 3737–TIL were cocultured for 6 hours with autologous B cells pulsed overnight with wt ERBB2IP, mut ALK, or mut ERBB2IP 25–amino acid–long peptides. Flow cytometry was used to assess expression of Vβ22 and to detect intracellular production of IL-2, TNF, and IFN-γ in the CD4+ population. Pie charts display the percentage of Vβ22+ cells that expressed the indicated number of cytokines. Data are representative of at least three independent experiments. (B) TCR-Vβ deep sequencing of 3737-TIL and blood and tumors of patient 3737 at various times pre- and post-adoptive cell transfer with 3737-TIL. Data show the frequency of the two ERBB2IP mutation–specific TCRβ-CDR3 clonotypes. ⊗, not detected.