Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 7;4(4):e00374-19. doi: 10.1128/mSphere.00374-19

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Moro-Bulnes et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

PMC Copyright notice

FIG 4 — TbCDA and TbDHFR-TS intracellular localization. Immunofluorescence analysis showing the subcellular localization of TbCDA in PF (A) and BF (B) parental cell lines, BFs and PFs overexpressing the enzyme (T. brucei BF CDA-OE and PF CDA-OE, respectively), and PFs overexpressing TbCDA-c-myc (T. brucei PF CDA-cmyc). (C) Immunofluorescence analysis of TbDHFR-TS localization in BFs and PFs. The signal was detected with either anti-TbCDA or anti-TbDHFR-TS and Alexa Fluor 488-conjugated goat anti-rabbit or anti-c-myc and Alexa Fluor 488-conjugated goat anti-mouse antibodies. Mitochondria were stained with MitoTracker Red CMXRos, and nuclei and kinetoplasts were stained with DAPI. Images were captured using an Olympus IX81 microscope and deconvolved with Huygens Essential software (version 3.3; Scientific Volume Imaging). Images were analyzed by using Fiji software. DIC, differential interference contrast. Bar, 5 μm.