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Kinetic parameters for different substrates of TbCDA determined at 282 nm
| Substrate | TbCDA |
||||
|---|---|---|---|---|---|
| Km (μM) |
Vmax
(μmol·min−1·mg−1) |
Kcat (min−1) |
Kcat/Km
(μM−1·min−1) |
Vmax/Km | |
| Deoxycytidine | 52.3 ± 4.5 | 34.9 ± 0.8 | 745.6 ± 17.6 | 14.3 | 0.67 |
| Cytidine | 199.5 ± 9.0 | 126.1 ± 3.4 | 2,690.4 ± 72.0 | 13.5 | 0.63 |
| 5-Methyl-2′-deoxycytidine | 24.5 ± 3.3 | 5.9 ± 0.2 | 125.6 ± 4.8 | 5.1 | 0.24 |
