FIG 3.

Karyotype polymorphism between wild-type strain and ΔmshA mutants of A. fumigatus. (A) Chromosomal bands separated by PFGE and stained with ethidium bromide. The gel on the left shows the chromosome profile of the A. fumigatus wild type. The profiles of the mutants were obtained in another gel shown at the right side of the figure panel (see the raw image in Fig. S2). The A. fumigatus wild type and mutants have five chromosomal bands. The smallest band (∼1.0 Mb) seen in the wild type and mutants might correspond to a minichromosome. S. pombe and S. cerevisiae chromosomal bands were used as size standards indicated in megabases (Mb). (B) Diagrammatic representation of karyotypes of the A. fumigatus wild type and mutants. The rectangles represent a unique distinguishable band visualized after staining with ethidium bromide. The thickness of the rectangles represents the proportional level of staining of each chromosomal band. The in silico chromosomes of A. fumigatus assigned to the chromosomal bands are indicated at the left.