TABLE 3.
Strains
| Species and strain | Description | Reference or source |
|---|---|---|
| B. thuringiensis | ||
| AW43 |
B. thuringiensis subsp. kurstaki HD73 cured of both pAW63 and pHT73, Nalr |
52 |
| THE2549 | AW43 thrC::PsigP-lacZ | This study |
| EBT140 | AW43 thrC::PsigP-lacZ ΔrasP | This study |
| EBT232 | AW43 thrC::PsigP-lacZ ΔsigP-rsiP | This study |
| EBT215 | AW43 thrC::PsigP-lacZ Δbla1 | This study |
| EBT360 | AW43 thrC::PsigP-lacZ/pAH9 Ptet-gfp-rsiP | This study |
| EBT366 | AW43 thrC::PsigP-lacZ ΔrasP/pAH9 Ptet-gfp-rsiP | This study |
| EBT510 | AW43 thrC::PsigP-lacZ ΔrasP/pAH9 Ptet-gfp-rsiP1–53 | This study |
| EBT516 | AW43 thrC::PsigP-lacZ/pAH9 Ptet-gfp-rsiP1–72 | This study |
| EBT518 | AW43 thrC::PsigP-lacZ/pAH9 Ptet-gfp-rsiP1–53 | This study |
| EBT533 | AW43 thrC::PsigP-lacZ ΔrasP/pAH9 Ptet-gfp-rsiP1–72 | This study |
| EBT175 | AW43 thrC::PsigP-lacZ ΔrasP/pAH9 | This study |
| EBT176 | AW43 thrC::PsigP-lacZ ΔrasP/pAH9 rasP | This study |
| EBT238 | AW43 thrC::PsigP-lacZ ΔsigP-rsiP/pAH9 PsigP-sigP-rsiP | This study |
| EBT251 | AW43 thrC::PsigP-lacZ ΔsigP-rsiP/pAH9 | This study |
| THE2642 | AW43 thrC::PsigP-lacZ rsiP1–16 | This study |
| THE2637 | AW43 thrC::PsigP-lacZ rsiP1–61 | This study |
| THE2628 | AW43 thrC::PsigP-lacZ rsiP1–80 | This study |
| THE2602 | AW43 thrC::PsigP-lacZ rsiP1–220 | This study |
| THE2605 | AW43 thrC::PsigP-lacZ ΔrasP rsiP1–16 | This study |
| EBT133 | AW43 thrC::PsigP-lacZ ΔrasP rsiP1–61 | This study |
| EBT148 | AW43 thrC::PsigP-lacZ ΔrasP rsiP1–80 | This study |
| EBT116 | AW43 thrC::PsigP-lacZ ΔrasP rsiP1–220 | This study |
| EBT567 | AW43 thrC::PsigP-lacZ rsiP1–16/pAH9 PsigP-sigP-rsiP | This study |
| EBT566 | AW43 thrC::PsigP-lacZ rsiP1–61/pAH9 PsigP-sigP-rsiP | This study |
| EBT565 | AW43 thrC::PsigP-lacZ rsiP1–80/pAH9 PsigP-sigP-rsiP | This study |
| EBT564 | AW43 thrC::PsigP-lacZ rsiP1–220/pAH9 PsigP-sigP-rsiP | This study |
| EBT168 | AW43 thrC::PsigP-lacZ/pAH9 PsigP-sigP-rsiP | This study |
| EBT169 | AW43 thrC::PsigP-lacZ pAH9 | This study |
| EBT563 | AW43 thrC::PsigP-lacZ rsiP1–16/pAH9 | This study |
| EBT562 | AW43 thrC::PsigP-lacZ rsiP1–61/pAH9 | This study |
| EBT561 | AW43 thrC::PsigP-lacZ rsiP1–80/pAH9 | This study |
| EBT560 | AW43 thrC::PsigP-lacZ rsiP1–220/pAH9 | This study |
| UM20 | AW43/pAH13 | This study |
| EBT587 | AW43 thrC::PsigP-lacZ rsiP1–80/pAH9 Ptet-gfp-rsiP | This study |
| E. coli | ||
| OmniMax 2-T1R | F′ {proAB+
lacIq
lacZΔM15 Tn10(Tetr) Δ(ccdAB)} mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80(lacZ)ΔM15 Δ(lacZYA-argF)U169 endA1 recA1 supE44 thi-1 gyrA96 relA1 tonA panD |
Invitrogen |
| INV110 |
endA1 rpsL thr leu thi lacY galK galT ara tomA tsx dam dcm supE44 Δ(lac-proAB) [F′ traD36 proAB lacIqZΔM15] |
Invitrogen |