TABLE 4.
Plasmids
| Plasmid | Relevant feature(s) | Parent vector | Digestion enzymes | Insert primers | Reference |
|---|---|---|---|---|---|
| pMAD | ori-pE194ts | 56 | |||
| pAH9 | ori-pE194 PsarA-mcherry | 57 | |||
| pAH13 | Ptet-gfp | 57 | |||
| pRAN332 | Ptet-gfp | 64 | |||
| pEBT4 | ori-pE194ts, ΔblaP | pMAD | BgIII, EcoRI | 3832 and 3833, 3834 and 3835 | This study |
| pEBT5 | ori-pE194ts, ΔrasP | pMAD | BgIII, EcoRI | 3632 and 3633, 3634 and 3635 | This study |
| pEBT6 | ori-pE194ts, ΔsigP-rsiP | pMAD | BgIII, EcoRI | 3776 and 3777, 3778 and 3779 | This study |
| pEBT13 | Ptet-gfp-rsiP | pAH9 | HindIII, EcoRI | 3838 and 3839 | This study |
| pCE630 | Ptet-gfp-rsiP1–72 | pAH9 | HindIII, EcoRI | 3838 and 4258 | This study |
| pCE632 | Ptet-gfp-rsiP1–53 | pAH9 | HindIII, EcoRI | 3838 and 4259 | This study |
| pTHE960 | PsigP-sigP+-rsiP+ | pAH9 | HindIII, EcoRI | 3774 and 3775 | This study |
| pIA02 | PsarA-rasP+ | pAH9 | EcoRI, KpnI | 3744 and 3745 | This study |
| pTHE946 | pE194ts | pMAD | BamHI, StuI | This study | |
| pTHE948 | pE194ts ‘thrC thrB’ | pTHE946 | ScaI, SalI | 2917 and 2918, 2919 and 2920 | This study |
| pTHE950 | pE194ts ‘thrC lacZ thrB’ | pTHE948 | XhoI, SbfI | 2922 and 2923 | This study |
| pTHE949 | pE194ts ‘thrC PsigP-lacZ thrB’ | pTHE950 | XhoI, SalI | 2929 and 2930 | This study |