Table 1.
Protein information, conserved amino acid residues, transmembrane domains (TMDs), and their predicated subcellular localization of Cucumis sativus aquaporins
| Name | Gene ID | aLength | bMW | cpI | dGRAVY | eTM | Ar/R selectivity filter | NPA motifs | Froger’s positions | fLoc | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| H2 | H5 | LE1 | LE2 | LB | LE | P1 | P2 | P3 | P4 | p5 | ||||||||
| CsPIP1;1 | Csa5M199270.1 | 292 | 31.43 | 7.67 | 0.31 | 6 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
| CsPIP1;2a | Csa5M198770.1 | 292 | 31.37 | 7.68 | 0.34 | 6 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
| CsPIP1;2b | Csa5M198770.2 | 217 | 23.20 | 7.62 | 0.21 | 4 | F | / | / | / | NPA | / | Q | / | / | / | / | plas |
| CsPIP1;3 | Csa5M153020.1 | 286 | 30.76 | 9.23 | 0.36 | 6 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
| CsPIP1;4 | Csa5M199280.1 | 292 | 31.40 | 7.67 | 0.29 | 5 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
| CsPIP1;5 | Csa6M445090.1 | 191 | 20.26 | 9.51 | 0.47 | 4 | / | N | A | R | NPA | NPA | K | S | A | F | W | plas |
| CsPIP1;6 | Csa3M739030.1 | 290 | 31.25 | 9.10 | 0.49 | 6 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
| CsPIP2;1a | Csa6M445130.1 | 284 | 29.92 | 7.04 | 0.51 | 6 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
| CsPIP2;1b | Csa6M445130.2 | 208 | 21.92 | 6.18 | 0.45 | 4 | F | S | / | / | NPA | / | Q | / | / | / | / | plas |
| CsPIP2;2 | Csa6M445120.1 | 284 | 30.24 | 8.78 | 0.56 | 6 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
| CsPIP2;3a | Csa6M445140.1 | 284 | 29.98 | 7.64 | 0.56 | 7 | F | A | F | T | NPA | HLA | Q | I | G | F | N | plas |
| CsPIP2;3b | Csa6M445140.2 | 208 | 21.93 | 6.26 | 0.56 | 4 | F | S | / | / | NPA | / | Q | / | / | / | / | plas |
| CsPIP2;4a | Csa6M140850.1 | 283 | 30.24 | 7.63 | 0.47 | 6 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
| CsPIP2;4b | Csa6M140850.2 | 252 | 26.85 | 6.06 | 0.47 | 5 | F | H | T | R | NPA | NPA | Q | S | A | S | L | plas |
| CsPIP2;5 | Csa6M405320.1 | 276 | 29.37 | 9.36 | 0.53 | 6 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
| CsPIP2;6a | Csa6M445150.1 | 279 | 29.72 | 8.97 | 0.62 | 6 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
| CsPIP2;6b | Csa6M445150.2 | 197 | 20.96 | 9.75 | 0.69 | 5 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
| CsPIP2;7 | Csa5M623360.1 | 287 | 30.52 | 9.11 | 0.41 | 6 | F | H | T | R | NPA | NPA | Q | S | A | F | W | plas |
| CsPIP2;8 | Csa7M014450.1 | 280 | 29.85 | 9.24 | 0.45 | 6 | F | H | T | R | NPA | NPA | M | S | A | F | W | plas |
| CsTIP1;1 | Csa6M448110.1 | 250 | 25.72 | 5.64 | 0.78 | 7 | H | I | A | V | NPA | NPA | F | A | S | Y | W | vacu |
| CsTIP1;2 | Csa3M743400.1 | 253 | 26.29 | 6.03 | 0.93 | 7 | H | I | A | V | NPA | NPA | F | A | A | Y | W | vacu |
| CsTIP1;3 | Csa5M505790.1 | 254 | 26.53 | 5.30 | 0.71 | 6 | H | I | A | V | NPA | NPA | F | T | A | Y | W | vacu |
| CsTIP2;1 | Csa5M162580.1 | 250 | 25.09 | 5.39 | 0.99 | 6 | H | I | G | R | NPA | NPA | F | S | A | Y | W | plas |
| CsTIP2;2 | Csa7M447100.1 | 248 | 25.44 | 5.66 | 0.87 | 6 | H | I | G | R | NPA | NPA | Y | S | A | Y | W | vacu |
| CsTIP3;1 | Csa1M043290.1 | 289 | 30.66 | 7.17 | 0.46 | 5 | H | I | A | R | NPA | NPA | L | A | S | Y | W | vacu |
| CsTIP4;1 | Csa2M374630.1 | 247 | 25.72 | 6.01 | 0.90 | 7 | H | I | A | R | NPA | NPA | Y | S | A | Y | W | vacu |
| CsTIP5;1 | Csa5M168860.1 | 255 | 26.12 | 6.88 | 0.86 | 6 | N | V | G | C | NPA | NPA | A | A | A | Y | W | plas |
| CsNIP1;1 | Csa6M520340.1 | 276 | 29.45 | 9.58 | 0.54 | 6 | W | V | A | R | NPA | NPA | F | S | A | Y | I | plas |
| CsNIP1;2 | Csa3M345890.1 | 269 | 28.88 | 7.67 | 0.78 | 5 | W | V | A | R | NPA | NPA | F | S | A | Y | M | vacu |
| CsNIP2;1 | Csa3M826640.1 | 288 | 30.55 | 9.40 | 0.33 | 6 | G | S | G | R | NPA | NPV | L | T | A | Y | F | vacu/plas |
| CsNIP2;2 | Csa3M826650.1 | 261 | 27.62 | 6.05 | 0.45 | 6 | C | S | G | R | NPA | NPA | M | S | A | Y | M | plas |
| CsNIP3;1a | Csa5M146200.1 | 249 | 26.01 | 8.86 | 0.65 | 4 | A | I | G | R | NPS | NPV | F | T | A | Y | M | plas |
| CsNIP3;1b | Csa5M146200.2 | 221 | 23.51 | 8.50 | 0.74 | 6 | A | I | / | / | NPS | / | F | / | / | F | l | plas |
| CsNIP3;2 | Csa5M146190.1 | 298 | 30.80 | 8.63 | 0.50 | 5 | A | I | G | R | NPS | NPV | F | A | A | Y | l | plas |
| CsNIP3;3 | Csa4M007030.1 | 304 | 31.52 | 8.26 | 0.43 | 5 | T | V | A | R | NPA | NPV | F | T | A | Y | l | plas |
| CsNIP4;1 | Csa3M149960.1 | 268 | 28.45 | 6.88 | 0.68 | 6 | A | V | A | R | NPA | NPA | F | S | A | Y | I | plas/vacu |
| CsSIP1;1 | Csa4M192210.1 | 243 | 25.59 | 9.41 | 0.83 | 6 | V | T | P | N | NPT | NPA | F | A | A | Y | W | vacu |
| CsSIP2;1 | Csa3M816140.1 | 238 | 25.93 | 10.00 | 0.66 | 5 | F | K | G | S | NPL | NPA | F | V | A | Y | W | vacu |
| CsXIP1;1 | Csa2M263850.1 | 319 | 34.47 | 8.27 | 0.66 | 7 | V | I | V | R | SPI | SPA | Y | C | A | F | W | cyto |
| / | Csa6M445100.1 | 106 | 11.57 | 4.51 | 0.62 | 2 | F | / | / | / | / | / | / | / | / | / | / | plas |
| / | Csa7M336420.1 | 99 | 10.66 | 4.83 | 0.72 | 3 | / | / | / | / | / | / | / | / | / | / | / | mito |
aLength Protein length (aa)
bMW Protein molecular weight (kD)
cpI isoelectric point
dGRAVY Grand average of hydrophobicity
eTM represents for the numbers of Transmembrane helices predicted by TMHMM Server v.2.0 tool
fBest possible cell localization predicted by the WoLF PSORT tool (Chlo Chloroplast, Cyto Cytosol, Plas Plasma membrane, Vacu Vacuolar membrane)
LB Loop B, LE Loop E. NPA Asparagine-Proline-Alanine, H2 represents for Helix 2, H5 represents for Helix 5, LE1 represents for Loop E1, LE2 represents for Loop E2, Ar/R represents for Aromatic/Arginine