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. 2019 Aug 8;24:51. doi: 10.1186/s11658-019-0177-6

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — The levels of ALP and calcification after HIF1AN overexpression or knockdown during osteoblast differentiation of MC3T3-E1 cells after incubation in DM supplemented with BMP2 for 14 days. a and b – The expression of HIF1AN following MC3T3-E1 cells being transfected with HIF1AN pcDNA3.1 or pcDNA3.1 was measured via quantitative RT-PCR (a) and western blot (b). ***p < 0.001 vs. pcDNA. c – The level of ALP was measured using an ALP assay kit. d – The area stained with alizarin red staining was quantified. e – The level of calcification was measured using alizarin red staining. ***p < 0.001 vs. control; ^###p < 0.001 vs. pcDNA; ^△△p < 0.01, ^△△△p < 0.001 vs. pcDNA-HIF1AN + miR-135-5p