Fig. 4.

Phenotypes of T-cells expanded in the Quantum system. a CD4⁺/CD8⁺ T-cell ratios. Percentages of the CD3⁺ T-cell parent population are shown. Cell populations are shown for pre-seeding (pre) and after harvest (post) for T-cells expanded from PBMCs of 5 healthy donors. b Gating strategy used to identify naive and memory T-cell phenotypes. One representative example is shown (donor 1, high seed). c Frequencies within the CD4⁺ and CD8⁺ T-cell populations of TN (CD45RA⁺/CCR7⁺), TCM (CD45RO⁺/CCR7⁺), TEM (CD45RO⁺/CCR7⁻) and TE (CD45RA⁺/CCR7⁻) subsets. Data are expressed as % of CD4⁺ or CD8⁺ T-cell populations. d Gating strategy used to analyze the expression of exhaustion markers LAG-3, PD-1 and Tim-3 on CD4⁺ and CD8⁺ T-cells. One representative example is shown (donor 1). e Frequencies of CD4⁺ and CD8⁺ T-cells expressing exhaustion markers. f MFI for expression of exhaustion markers on positively-gated events for CD4⁺ and CD8⁺ T-cells. Error  bars represent one standard deviation from the mean. Pre pre-expansion PBMCS, LS low seed, HS high seed