Fig. 3.

Morphology of and marker expression in hiPSCs and trophoblast cells derived from cysts (TS^hiPSC). a Phase-contrast images of hiPSCs and TS^hiPSC cells. Scale bar = 100 μm. b, c Bright-field and immunofluorescence images of hiPSCs and TS^hiPSC cells stained for POU5F1, NANOG, SOX2, and rBC2LCN (b) and GATA3, TP63, and KRT7 (c). Nuclei were stained with Hoechst 33342. Scale bar = 100 μm. d Analysis of pluripotency and trophoblast gene expression by qRT-PCR in hiPSCs and TS^hiPSC cells grown on laminin-coated dishes for 3 days. Expression levels were calculated relative to those of GAPDH and normalized to those of control hiPSCs. Values are the means ± SEMs (n = 4). Significance of differences was determined by Student’s t tests. *P < 0.05 versus the hiPSC group. e Flow cytometry histograms of KRT7 expression in TS^hiPSC cells. Similar results were obtained for three independent cell samples