Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 7;10:245. doi: 10.1186/s13287-019-1339-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 5 — Directed differentiation of TS^hiPSC cells into STB- and EVT-like cells. a Phase-contrast image of TS^hiPSC cells containing multinucleated STB-(2D) cells (yellow-dotted line area). b Immunofluorescence images of TS^hiPSC cells containing multinucleated STB-(2D) cells stained for E-cadherin and hCG; nuclei were stained with Hoechst 33342. c Changes in hCG secretion by TS^hiPSC cells over 5 days. Data are presented as means ± SEMs (n = 4). Significance of differences was determined by one-way analysis of variance with repeated measures. d Phase-contrast images of STB-(3D) cells in low-adherence culture dishes on days 0 and 6. e Immunofluorescence images of TS^hiPSC cells and STB-(3D) cells stained for hCG. f Immunofluorescence images of STB-(3D) cells at z-position, showing the STB-(3D) cyst structure, which had several clear interior cavities and a thin enclosing wall. g Analysis of pluripotency and trophoblast gene expression by qRT-PCR in TS^hiPSC and STB-(3D) cells. Expression levels were calculated relative to those of GAPDH and normalized to those of TS^hiPSC cells. Values are the means ± SEMs (n = 4). Significance of differences was determined by Student’s t tests. *P < 0.05 versus the TS^hiPSC cell group. h Levels of hCG secreted by TS^hiPSC and STB-(3D) cells. For comparison, both TS^hiPSC and STB-(3D) cells were cultured in low-adherence Petri dishes. Data are presented as means ± SEMs (n = 3). i Phase-contrast images of TS^hiPSC and EVT cells. EVT cells had a mesenchyme-like morphology. The arrows indicate the presence of pseudopodia on EVT cells. j Immunofluorescence images of TS^hiPSC and EVT cells stained for HLA-G. k Analysis of TP63, HLA-G, and CGB gene expression by qRT-PCR in TS^hiPSC and EVT cells. Expression levels were calculated relative to those of GAPDH and normalized to those in TS^hiPSC cells. Values are the means ± SEMs (n = 4). Significance of differences was determined by Student’s t tests. *P < 0.05 versus the TS^hiPSC cell group. Unless otherwise noted, the scale bars in all phase-contrast and immunostaining images are 100 μm