Fig. 4.

Effects of SNORD89 interference on cell proliferation and self-renewal ability of ovarian cancer cells. a The correlation analysis of SNORD89 expression with other genes expression using the data from ovarian cancer patients in TCGA. b The flow cytometry analysis of the proportion of cell cycle phases in OV cells transfected with SNORD89 OE plasmids. c The flow cytometry analysis of the proportion of cell cycle phases in OS cells transfected with shRNA-1 and shRNA-2 plasmids. d The cell proliferation was measured in OV cells of SNORD89 overexpression by Cell Counting Kit-8 (CCK-8) assays at 24 h, 48 h, 72 h and 96 h transfection. e The cell proliferation was measured in OS cells of SNORD89 silence by CCK-8 assays at 24 h, 48 h, 72 h and 96 h transfection. f The cell proliferation ability was measured in OV cells of SNORD89 overexpression by plate clone formation assay. g The cell self-renewal ability was measured in OV cells of SNORD89 overexpression by soft agar colony formation assay. h The cell self-renewal ability was measured in OS cells of SNORD89 silence by colony formation assays