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. 2019 Aug 8;17:259. doi: 10.1186/s12967-019-2005-1

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Effects of SNORD89 interference on the expression of c-Myc and Notch1 of ovarian cancer cells. a The mRNA expression of c-Myc and Notch1 was detected in OV and OS cells by qRT-PCR. The mRNA expression of the two genes in OV cells was set as 1. b The representative agarose gel electrophoresis photos showed the expression of c-Myc and Notch1 in OV and OS cells after RT-PCR. GAPDH was used an endogenous control. c The mRNA expression of c-Myc and Notch1 was detected in OV cells transfected with SNORD89 OE or NC plasmids at 24 h by qRT-PCR. The mRNA expression of the two genes in OV cells transfected with NC plasmids was set as 1. d The representative agarose gel electrophoresis photos showed the increased expression of c-Myc and Notch1 in OV cells transfected with SNORD89 OE. e The mRNA expression of c-Myc and Notch1 was detected in OS cells transfected with shRNA-1, shRNA-2, and shRNA-NC plasmids at 48 h by qRT-PCR. f The representative agarose gel electrophoresis photos showed the decreased expression of c-Myc and Notch1 in OS cells transfected with SNORD89 shRNA-1 and shRNA-2. g The representative western blot photos showed the increased expression of c-Myc and Notch1 in OV cells transfected with SNORD89 OE. h The representative western blot photos showed the decreased expression of c-Myc and Notch1 in OS cells transfected with SNORD89 shRNA-1 and shRNA-2. β-actin was used an endogenous control