Fig. 4.

Effects of the CYP4A inhibitor HET0016 in a novel 3D hepatic steatosis model. a Gross morphology of control spheroids, glucose/palmitate-treated steatosis spheroids, and HET0016-treated steatosis spheroids. b ALB production was determined in each indicated spheroid normalized to ATP concentration; n = 3. c mRNA and d Protein expression levels of gluconeogenesis- and lipogenesis-related genes were determined in each indicated spheroid; n = 3. β-Actin was used as an internal control for mRNA and protein expression. e Intracellular lipids were stained with Oil red O (top) and Nile red (bottom) and quantified by Nile red fluorescence intensity (right); n = 3. f The triglyceride concentration was quantified by absorbance in each indicated spheroid; n = 3. g Glucose uptake was quantified by fluorescence intensity. **p < 0.01 and ***p < 0.001