Table 3.

Genes differentially expressed between predators and nonpredators in de novo Chrysomya rufifacies transcriptomes

	No.	Name	Experimental function	Predicted function	Effects in Drosophila
↑	20	Host cell factor	Chromatin binding; contributes to histone acetyltransferase activity	Sequence‐specific DNA binding transcription factor activity; transcription coactivator activity	activation and the regulation of cellular growth (Furrer et al., 2010; Mahajan, Johnson, & Wilson, 2003). component of regulatory networks related to osmotic stress (Suganuma et al., 2010)
↑	20	Arginase		Arginase activity; metal ion binding	catabolize arginine in Drosophila (arg) (Sardiello, Licciulli, Catalano, Attimonelli, & Caggese, 2003) putative genetic marker of aggressive Drosophila (Edwards, Rollmann, Morgan, & Mackay, 2006)
↑	18	AMP‐activated protein kinase α subunit	AMP‐activated protein kinase activity	ATP binding; G protein‐coupled receptor kinase activity; protein serine/threonine kinase activity	part of the Target of Rapamycin (TOR) signaling pathway (Bland et al., 2010; Stroschein‐Stevenson et al., 2006) nutrition‐state dependent behavior and physiology (fat body signaling, smooth muscle function, nutrient absorption; Bland et al., 2010; Johnson et al., 2010; Stroschein‐Stevenson et al., 2006) role in dendrite morphogenesis (Swick et al., 2013)
↑	16	Silver	Carboxypeptidase activity; metallocarboxypeptidase activity	Metallocarboxypeptidase activity; serine‐type carboxypeptidase activity; zinc ion binding	contains carboxypeptidase D (CPD) domain (Sidyelyeva & Fricker, 2002), preference for C‐terminal arginase residues (Sidyelyeva, Baker, & Fricker, 2006) expressed in trans‐Golgi network (Varlamov & Fricker, 1998) involved with secretory protein processing (Novikova et al., 1999; Song & Fricker, 1995; Varlamov & Fricker, 1998), specifically the dopaminergic pathway; mutants have increased N‐acetyldopamine levels (Walter et al., 1996)
↑	14	Transport and Golgi organization 5			associated with endoplasmic reticulum and Golgi apparati (Bard et al., 2006), conserved across kingdoms (Rabouille & Kondylis, 2006; Wang et al., 2011) required for protein secretion (Rabouille & Kondylis, 2006) expressed in the central nervous system of immature Diptera (dos Santos et al., 2015)
↓	11	Asterix			classically known for role in gametogenesis (Dönertas et al., 2013) required for Piwi‐piRNA (Piwi‐interacting RNA) complex silencing of transposons in the female germ line (Dönertas et al., 2013) highest expression in larvae localized to fat body (dos Santos et al., 2015)
↑	10	Glass bottom boat		Growth factor activity; transforming growth factor beta receptor binding	part of the Bone Morphogenetic Pathway (BMP; Akiyama et al., 2012; Ballard, Jarolimova, & Wharton, 2010) nutrition‐state dependent behavior and physiology; sensitivity to nutritionally available lipids (Ballard et al., 2010) neuron morphogenesis and proper function and morphology of the neuromuscular junction (Akiyama et al., 2012; Keshishian & Kim, 2004; McCabe et al., 2003)

This table summarizes the results of analysis of genes differentially expressed between actively predating and nonpredating C. rufifacies third instars across 24 de novo transcriptome assemblies limited to genes differentially expressed in at least 10 different assemblies. Columns (left to right): Upregulated (up arrows) or downregulated (down arrows) in predators, number of assemblies detected in (#), name of gene based on Drosophila melanogaster annotation (Name), experimental molecular function(s) (Experimental), predicted molecular function(s) (Predicted), and known effects and interactions of this gene or its homologues in other species (Effects).