FIGURE 6.

Effect of blockade of ionotropic glutamate receptors in DMH or inhibition of MnPO neurons on the BAT thermogenesis evoked by inhibition of vLPO neurons. A, bilateral nanoinjection of AP5/CNQX (straight line; 60 nL; AP5 and CNQX, 5 mmol/L each; n = 4) in the dorsomedial hypothalamus (DMH) terminate the raise of BAT SNA, BAT temperature (T_BAT), expired CO₂ (Exp CO₂), core temperature (T_CORE), mean arterial pressure (MAP), and heart rate (HR) evoked by bilateral nanoinjections of muscimol (1.2 mmol/L, 60 nL) in vLPO. B, composite mapping of the muscimol nanoinjection sites plotted on drawing of vLPO at −0.6 mm caudal to bregma; composite mapping of the AP5/CNQX nanoinjection sites plotted on drawing of DMH at −3.2 mm caudal to bregma; and histological section containing the fluorescent beads marking a representative nanoinjection. D, nanoinjection of muscimol (1.2 mmol/L, 60 nL; straight line; n = 4) in the median preoptic area (MnPO) does not affect the raise of BAT SNA, T_BAT, T_CORE, Exp CO₂, MAP, and HR evoked by bilateral nanoinjections of muscimol (1.2 mmol/L, 60 nL; dashed line; n = 4) in vLPO. E, composite mapping of the muscimol nanoinjection site s plotted on drawing of vLPO at −0.6 mm caudal to bregma; composite mapping of the muscimol nanoinjection sites plotted on drawing of MnPO at 0.1 mm and −0.1 mm from bregma; and histological section containing the fluorescent beads marking a representative nanoinjection. ac, anterior commissure; DMH, dorsomedial hypothalamus; f, fornix; HDB, diagonal band of Broca; LPO, lateral preoptic; MnPO, median preoptic area; MPA, medial preoptic area; mt, mammillothalamic tract; oc, optic chiasm; 3 V, third ventricle; vLPO, ventral part of the lateral preoptic area