Figure 2. Effects of RAGE on Thermogenic Gene Expression during Fasting and Cold Challenge.

(A–D) Fasting and refeeding in 11-week-old male WT and Ager null mice.

(A) Ager expression in iBAT and iWAT from WT mice in a fed, fasted (24 h) or 24-h refed state.

(B) Change in core body temperature, [(Δ) temperature], between the fasted and the refed states was measured in WT or Ager null male mice housed at room temperature (RT).

(C and D) Relative mRNA expression of Acox1 and Cpt2a was measured by qRT-PCR in (C) iBAT and (D) iWAT from fed and fasted (24 h) WT and Ager null mice.

(E–I) Eight-week-old male WT or Ager null mice were exposed to a cold challenge (4°C).

(E and F) iBAT (E) and iWAT (F) from WT mice were subjected to qRT-PCR for relative Ager mRNA expression.

(G) (Δ) Temperature from baseline (room temperature) and after 48 h at 4°C is shown.

(H and I) WT or Ager null mice were housed at room temperature (~23°C) or exposed to a 4°C cold challenge for 6 or 12 h. iBAT (H) and iWAT (I) were dissected after and subjected to qRT-PCR for detection of relative Ucp1 mRNA expression.

In (B)–(D) and (G)–(I): WT (black bars) and Ager null (green bars) mice. Data are presented as mean ± SEM in N = 4–6 mice/group. Data analysis: (A, E, and F) one-way ANOVA with post hoc Tukey’s HSD test; (B and G) two-tailed Student’s t test; (C, D, H, and I) two-way ANOVA followed by a post hoc Sidak test or Tukey’s test, as appropriate; *p < 0.05; **p < 0.01, and ***p < 0.001.

See also Figure S2.