FIG. 4.

Microvascular networks maintain perfusion during culture. (A, B) Representative immunofluorescence images of capillary networks perfused with FITC-conjugated albumin (green) after 48 h in culture. The * symbol indicates newly formed capillary loops with perfusion culture. (C–F) Examples of newly formed capillary sprouts perfused with FITC-conjugated albumin (green). Microvascular networks were identified by lectin (red) labeling, where blood and lymphatic vessels were distinguished based on their morphology and network structure.