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Representative immunofluorescence images of perivascular cells after 48 h in perfusion culture. (A) αSMA-positive smooth muscle cells maintain tight wrapping morphologies along main arteriole and venule blood vessels in perfused microvascular networks. V, venule; A, arteriole. (B) NG2-positive (red) vascular pericytes maintain close wrapping along blood capillaries (green) in perfused microvascular networks. Arrows indicate individual vascular pericyte cell bodies.
