FIG. 6.

Evaluation of angiogenesis in microvascular networks cultured with flow (Perfused) and without flow (Static). Microvascular networks from both experimental Perfused (B) and Static (C) groups became angiogenic after 48 h in culture, defined by an increase in vascular density (D) and capillary sprouts (E) compared to microvascular networks from freshly harvested mesentery tissue in the Unstimulated (A) group (t = 0 h). Angiogenic microvascular networks cultured without flow exhibited significantly (p = 0.0027) denser vascular structures compared to networks cultured with flow. Interestingly, no significant difference (p = 0.9269) was observed in capillary sprouting between Perfused and Static groups. Black, white, and striped bars represent Unstimulated, Perfused, and Static groups, respectively. The *** and **** indicate a significant difference of p < 0.001 and p < 0.0001 by one-way ANOVA and Tukey's post hoc method comparing vascular density between Perfused and Static groups. The “ns” indicates no significant difference (p > 0.05) comparing capillary sprouts between Perfused and Static groups.