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. 2019 Aug 1;6:105. doi: 10.3389/fcvm.2019.00105

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Copyright © 2019 Stellato, Czepiel, Distler, Błyszczuk and Kania.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cardiac Lin⁻gp38⁺ cells represent collagen I producing cells. Representative flow cytometry analysis of cells isolated from hearts of Col1a1-EGFP tg mice. Cellular debris below 50 k in FSC-A were excluded in SSC-A/FSC-A plots. Live cells were first gated as Col1a1-EGFP⁺ or Col1a1-EGFP⁻ (A) and then analyzed for expression of Lin and gp38. The percentage of Lin⁻gp38⁺ cells was quantified from Col1a1-EGFP⁺ cells (B) and Col1α1-EGFP⁻ cells (C). (D) Shows quantification of Lin⁻gp38⁺ cell percentages from EGFP⁻ (white circles) and EGFP⁺ (green circles) cells (n = 5). (E) Shows representative staining of gp38 (red) and EGFP expression (green) indicating the collagen I-producing cells in the cryosections from hearts of Col1a1-EGFP tg mouse. DAPI stains cell nuclei. Bar = 50 μm.