Table 1. Photophysical properties and emission spectra of 2, 3, and 10 a , b , c .
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Cpd | λ max abs (nm) pH 7.2 | λ max abs (nm) pH 4.5 | λ max flu (nm) | FEF pH 7.4/4.5 d , e |
1 | 749 | 685 | 707 | 21 13a |
2 | 755 | 693 | 713 | 23 |
3 | 757 | 696 | 716 | 18 |
10 | 705 | 703 | 733 | 1.0 |
aDMEM/10% FBS solutions with 4 mM TX-100.
bTo allow a common comparison for each fluorophore solution across a wide acidity range 4 μM Triton X 100 was included in each solution.
cFluorescence spectra taken at 5 μM concentration, 2 (black spectra) 3 (grey spectra) 10 (red spectrum), inset shows expansion of spectra for 2 and 3.
dFluorescence enhancement factor (FEF).
eFluorescence quantum yields at pH 7.4 of 2 and 3 = 0.16 and 0.18 respectively (ESI).