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Human leukocyte antigen (HLA) targeted immunofluorescence staining. Explanted intervertebral disc (IVD) samples were stained by anti‐HLA‐ABC (green) and nuclei DAPI staining (blue). Nontreated (L6/7) and negative control (no primary antibody) showed no bright positive HLA‐ABC staining. Cryopreserved (off‐the‐shelf [OTS]‐LOW) cell transplantation treated disc showed clusters of HLA‐positive cells within the nucleus pulposus (NP). Scale bar represent 50 μm
