Figure 4.

Implantation of hypertrophic chondrocytes promotes bone formation. (A) Three‐dimensional microcomputed tomography (3D μCT) reconstructions of the posterior spine (L3‐L5) following sham surgery, implantation of iliac crest bone graft (ICBG), or implantation of fracture callus chondrocyte graft (FCCG). Median radiograph quantification (RQ) per mouse correlates visually with the amount of bone formation (B) longitudinal RQ of bone formation via blinded scoring of digital radiographs (Figure S1). Points represent mean score between 3 reviewers per mouse ±SD. N = 10 mice per experimental group. *Statistical significance between ICBG or FCCG and sham. **P < 0.01, ***P < 0.001, ****P < 0.0001. Alpha = 0.05. No statistical difference between experimental groups was detected at any time point. (C) in vivo fluorescent imaging of bone deposition using Osteosense 800