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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: AIDS. 2019 Jul 1;33(8):1293–1306. doi: 10.1097/QAD.0000000000002195

Figure 2. HIV-DNA quantification in myeloid cells sorted by FACS directly from PBMC of HIV+ART individuals.

Figure 2

Matched CD16- Mo, CD16+ Mo, CD1c+ DC, and CD4+ T-cells were sorted directly by FACS from PBMC of HIV+ART individuals (Table 1a) upon staining with CD3, CD4, CD8, CD56, CD19, CD16, HLA-DR and CD1c Abs, using the gating strategy illustrated in panel A. Post-sort cell purity was >98%, as depicted in panel B. Levels of early (left panel), late (middle panel) HIV reverse transcripts and integrated HIV-DNA (right panel) were quantified using nested real-time PCR (C), as detailed in Figure 1 legend. The limit of detection (3 HIV-DNA copies/test), indicated by the dotted lines, was assigned to any undetectable sample. Each PCR reaction was performed in triplicates of 105 cells per test. Each symbol represents one different HIV+ART individual (n=3).