. Author manuscript; available in PMC: 2019 Aug 8.

Published in final edited form as: Stem Cell Res. 2019 May 15;38:101461. doi: 10.1016/j.scr.2019.101461

Table 1.

Characterization and validation.

Classification	Test	Result	Data
Morphology	Photography	Normal	Fig. 1 Panel A
Phenotype	Immunocytochemistry	SOX2, OCT4, NANOG, SSEA-4	Fig. 1 Panel A
Phenotype	Flow cytometry	TRA-1–60 (99.94%); NANOG (99.41%)	Fig. 1 Panel D
Genotype	Karyotype (G-banding) and resolution	46XX, Resolution: 425–500	Fig. 1 Panel B
Identity	Microsatellite PCR (mPCR) OR	Not performed	N/A
Identity	STR analysis	16 sites tested, all sites matched	Available from the authors
Mutation analysis	Sequencing	SMPD1, p.L302P (c.905 T > C)	Fig. 1 Panel C
Mutation analysis	Southern Blot OR WGS	N/A	N/A
Microbiology and virology	Mycoplasma	Mycoplasma testing by luminescence. Negative	Supplementary Fig. S1
Differentiation potential	Teratoma formation	Teratoma with three germlayers formation. Ectoderm (neural epithelium); Mesoderm (cartilage); Endoderm (gut tissue)	Fig. 1 Panel F
Donor screening	HIV 1 + 2 Hepatitis B, Hepatitis C	N/A	N/A
Genotype additional info	Blood group genotyping	N/A	N/A
Genotype additional info	HLA tissue typing	N/A	N/A