Table 2.

Reagents details.

Antibodies used for immunocytochemistry/flow-cytometry
	Antibody	Dilution	Company Cat # and RRID
Pluripotency Markers	Mouse anti-SOX2	1:50	R&D systems, Cat# MAB2018, RRID:AB_358009
Pluripotency Markers	Rabbit anti-NANOG	1:400	Cell Signaling Technology, Cat# 4903, RRID:AB_10559205
Pluripotency Markers	Rabbit anti-OCT4	1:400	Thermo Fisher Scientific, Cat# A13998, RRID:AB_2534182
Pluripotency Markers	Mouse anti-SSEA4	1:1000	Cell Signaling Technology, Cat# 4755, RRID:AB_1264259
Secondary antibodies	Donkey anti-Mouse IgG (Alexa Fluor 488)	1:400	Thermo Fisher Scientific, Cat# A21202, RRID:AB_141607
Secondary antibodies	Donkey anti-Rabbit IgG (Alexa Fluor 594)	1:400	Thermo Fisher Scientific, Cat# A21207, RRID:AB_141637
Flow cytometry antibodies	Anti-Tra-1–60-DyLight 488	1:50	Thermo Fisher Scientific, Cat# MA1–023-D488X, RRID:AB_2536700
Flow cytometry antibodies	Anti-Nanog-Alexa Fluor 488	1:50	Millipore, Cat# FCABS352A4, RRID:AB_10807973
Flow cytometry antibodies	Mouse-IgM-DyLight 488	1:50	Thermo Fisher Scientific, Cat# MA1–194-D488, RRID:AB_2536969
Flow cytometry antibodies	Rabbit IgG-Alexa Fluor 488	1:50	Cell Signaling Technology, Cat# 4340S, RRID:AB_10694568
Flow cytometry antibodies	Mouse IgG3-FITC	1:50	Thermo Fisher Scientific, Cat# 11–4742-42, RRID:AB_2043894
Primers
	Target		Forward/Reverse primer (5′-3′)
Sev specific primers (RT-PCR)	Sev/181 bp		GGATCACTAGGTGATATCGAGC/ACCAGACAAGAGTTTAAGAGATATGTATC
Sev specific primers (RT-PCR)	KOS/528 bp		ATGCACCGCTACGACGTGAGCGC/ACCTTGACAATCCTGATGTGG
Sev specific primers (RT-PCR)	Klf4/410 bp		TTCCTGCATGCCAGAGGAGCCC/AATGTATCGAAGGTGCTCAA
Sev specific primers (RT-PCR)	C-Myc/523 bp		TAACTGACTAGCAGGCTTGTCG/TCCACATACAGTCCTGGATGATGATG
House-Keeping gene (RT-PCR)	GAPDH/197 bp		GGAGCGAGATCCCTCCAAAAT/GGCTGTTGTCATACTTCTCATGG
Targeted mutation analysis (PCR)	SMPD¼54 bp		CTGAAGGTGAGCACTGAAGG/TGGTGAGAAATCAGAGGCAG