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. Author manuscript; available in PMC: 2019 Aug 19.
Published in final edited form as: Science. 2019 Jul 19;365(6450):280–284. doi: 10.1126/science.aau6232

Figure 1. Function of a small LanB enzyme, TglB, found in P. syringae.

Figure 1.

(A) LanB enzymes glutamylate Ser/Thr residues and subsequently eliminate the glutamate to form dehydroamino acids. Small LanB proteins lack the elimination domain. Dha, dehydroalanine; Dhb, dehydrobutyrine. (B) Biosynthetic gene cluster in P. syringae encoding a small LanB. (C) Matrix-assisted laser desorption ionization with time-of-flight (MALDI-TOF) mass spectra of TglA coexpressed with TglB. (D) Analysis of the TglB product by tandem electrospray ionization (ESI) mass spectrometry.