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. Author manuscript; available in PMC: 2019 Aug 8.
Published in final edited form as: Cell Rep. 2019 Jul 23;28(4):864–876.e6. doi: 10.1016/j.celrep.2019.06.080

Figure 4. Neuromuscular Synapse Formation Induces Ribosomal Decrease in Distal Axons.

Figure 4.

(A) Schematic representation of the co-culture system in microfluidic chambers. Spinal motor neurons (MNs), C2C12 muscle fibers, or HEK293 cells (control) were cultured in microfluidic chambers compartments according to the timeline. At DIV 4, MNs were transduced with a Sindbis virus expressing EGFP-L10a for 24 h.

(B) Nerve-muscle interaction causes SV accumulation and exogenous RibP L10a decrease. At DIV 5, co-cultures were immunostained against synapsin (red) and EGFP (green). Scale bar is 100 μm.

(C and D) Quantitative data of the puncta number of synapsin (C) and RibP L10a (D) clusters per axonal length. Bars represent the mean ± SEM. Statistical significance by unpaired Student’s t test.