Figure 1.

“Edge Assay” can measure locomotor ability of worms (A) (Left) Schematic diagram of an Edge Assay plate immediately after placing worms at the center of the plate. (Right) Schematic diagram of Edge Assay plate after most worms reached the edge. (B) Edge Assay completion rates of wild-type worms from adult day 1 to 7 after 5, 10, 15, 30, and 60 min. (C) Completion rates for WT and developmental mutants deficient in locomotor function, unc-54(e190), unc-43(e408), and unc-13(e51). (D) Completion rates of WT and a previously reported C. elegans model of amyotrophic lateral sclerosis (SOD1^127X). For Edge Assay experiments, n = 3 biological replicate plates with each plate starting with approximately 100 worms per plate on adult day 1. Error bars indicate 95% confidence intervals. *P < 0.05; Unpaired Student’s t-test.