Figure 5.

Superloser lowers 5-FOA^R mutants and increases isolation of humanized yeast. (a) Log10 frequency of 5-FOA^R colonies calculated as the number of colonies per plate divided by the total number of cells plated (∼1x10⁷ cells per OD600). G/R (Galactose/Raffinose); D (Dextrose). N = 20 for each condition, Two-tailed unpaired t-test. (b) PCRtag analysis of colonies emerging after 3 weeks of growth. Top, example PCRs from either a successful humanization event and an unsuccessful event, as indicated by the retention of the yeast histone genes. Bottom, summary results from the PCRtag analysis.