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. 2019 Jun 11;9(8):2629–2635. doi: 10.1534/g3.119.400399

Table 3. Amplicon repair template primers. Forward and reverse primer sequences used to make amplicons noted in each figure legend. Note some primers were used to generate more than one amplicon.

Name Primer Sets Template Product
Figure 2 Amplicon 1 F: cataaaactttgaccttgtgaagtgtcaaccttgaagtgattatagtctctgttttcg pCFJ70 Pmyo-3
(2.1) R:ctcaccattaagcctgcttttttgtacaaacttgtcaattctagatggatctagtggtcg
Figure 2 Amplicon 2 F: acaagtttgtacaaaaaagcaggcttaatggtgagcaagggcgaggagctg T5V - pTurquoise_5aa_Venus Venus
(2.2) R: gtacaagaaagctgggtactagatccggtggatcccgg
Figure 2 Amplicon 3 F: ccgggatccaccggatctagtacccagctttcttgtac pCFJ90 unc-54 UTR
(2.3) R:gctcaattcgcgccaaactatggtggttcgacagaaacagttatgtttggtatattg
Figure 2 Amplicon 4 F:tcagtgcagtcaacatgtcgagtttcgtgccgaatagtgattatagtctctgttttcgttaattttg pCFJ70 Pmyo-3
(2.4) R:ctcaccattaagcctgcttttttgtacaaacttgtcaattctagatggatctagtggtcg
Figure 2 Amplicon 5 F: ccgggatccaccggatctagtacccagctttcttgtac pCFJ90 unc-54 UTR
(2.5) R: aaacagaaaattaatactgtccgcgtttgctctttaacagttatgtttggtatattg
Figure 2 Amplicon 6 F:aaagacgatgagttctactggcgtggaatccacaaagtgattatagtctctgttttcg pCFJ70 Pmyo-3
(2.6) R:ctcaccattaagcctgcttttttgtacaaacttgtcaattctagatggatctagtggtcg
Figure 2 Amplicon 7 F: ccgggatccaccggatctagtacccagctttcttgtac pCFJ90 unc-54 UTR
(2.7) R: cgctagctacacatttttcccatctctcgggcaataacagttatgtttggtatattg
Figure 3 Amplicon 1 F:tcagtgcagtcaacatgtcgagtttcgtgccgaatcattttatatctgagtagtatcctttgc pCFJ90 Pmyo-2
(3.1) R: ttactcattaagcctgcttttttgtacaaacttgt
Figure 3 Amplicon 2 F:acaagtttgtacaaaaaagcaggcttaatgagtaaaggagaagaac pCZGY1614 GFP
(3.2) R:aaacagaaaattaatactgtccgcgtttgctctttttatttgtatagttcatccatg
Figure 3 Amplicon 3 F:acaagtttgtacaaaaaagcaggcttaatgagtaaaggagaagaac pCZGY1614 GFP
(3.3) R:tacaagaaagctgggtattatttgtatagttcatccatg
Figure 3 Amplicon 4 F: Gatgaactatacaaataatacccagctttcttgtac pCFJ90 unc-54 UTR
(3.4) R: aaacagaaaattaatactgtccgcgtttgctctttaacagttatgtttggtatattg
Figure 3 Amplicon 5 F:acaagtttgtacaaaaaagcaggcttaatgagtaaaggagaagaac pCZGY1614 GFP
(3.5) R:gagaccatcgatgctcctgaggctcccgatgctcctttgtatagttcatccatgcc
Figure 3 Amplicon 6 F:ggagcatcgggagcctcaggagcatcgatggtctcaaagggtgaagaag pCFJ90 Cherry
(3.6) R:ccgatgcggagctcagatatcacccactttgtaca
Figure 3 Amplicon 7 F: tgtacaaagtgggtgatatctgagctccgcatcgg pCFJ90 unc-54 UTR
(3.7) R: aaacagaaaattaatactgtccgcgtttgctctttaacagttatgtttggtatattg
Figure 4 Amplicon 1 F:tcagtgcagtcaacatgtcgagtttcgtgccgaatgacgacgacgacctcgacggcaac pSEP45 Prab-3
(4.1) R:gccatttttaagcctgcttttttgtacaaacttgtctgaaaatagggctactgtag
Figure 4 Amplicon 2 F:acaagtttgtacaaaaaagcaggcttaaaaatggctgcgttcttgctgagac pBB34 Sdhc::MAC
(4.2) R:ggatcctcctcctccagatcctcctccacctcgggcgccgtcgtcctcgccgatc
Figure 4 Amplicon 3 F:cccgaggtggaggaggatctggaggaggaggatccatggtttccgagttgatcaagg pKT133 mKate
(4.3) R:ttaacgatgtccgagcttggatgggagatcacaatatc
Figure 4 Amplicon 4 F:attgtgatctcccatccaagctcggacatcgttaagtccaattactcttcaac pCFJ90 unc-54 UTR
(4.4) R: aaacagaaaattaatactgtccgcgtttgctctttaaggtattttgtgtgcgg
Figure 4 Amplicon 5 F:tcagtgcagtcaacatgtcgagtttcgtgccgaatagcacagaactgcattaag pELA10 Pvha-6
(4.5) R:gccatttttaagcctgcttttttgtacaaacttgtatttttatgggttttggtag
Figure 4 Amplicon 6 F: tcagtgcagtcaacatgtcgagtttcgtgccgaatagtgattatagtctctgttttc pAYW7 Pmyo-3
(4.6) R: gccatttttaagcctgcttttttgtacaaacttgtcaattctagatggatctagtg