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. 2019 Jun 5;140(6):500–513. doi: 10.1161/CIRCULATIONAHA.119.041059

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© 2019 The Authors.

Circulation is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited.

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Figure 2. — HHIPL1 regulates human AoSMC migration and proliferation. A,HHIPL1 mRNA expression in human aortic smooth muscle cells (AoSMC), coronary artery endothelial cells (CAEC), peripheral blood mononuclear cells (PBMC), and macrophages (MP) relative to 36B4. B,HHIPL1 mRNA expression relative to RPLP0 in AoSMCs 24 to 72 hours after transfection. C,Migration rate of rate of human AoSMCs after siRNA transfection (left; n=3). Representative images of wound-healing assay (right). D, Number of AoSMCs over 72 hours after siRNA knockdown. E,Proportion of apoptotic cells 48 hours after knockdown. F, GLI1 and PTCH1 expression relative to RPLP0 48 hours after siRNA knockdown. G, Representative Western blot of GLI1 after siRNA knockdown. β-actin was used as a loading control. AoSMC indicates aortic smooth muscle cell; HHIPL1, hedgehog interacting protein-like 1; NTC, nontargeting control siRNA; and siRNA, small interfering RNA.