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. 2019 Jun 5;140(6):500–513. doi: 10.1161/CIRCULATIONAHA.119.041059

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© 2019 The Authors.

Circulation is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited.

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Figure 7. — Hhipl1 deficiency reduces smooth muscle cell content in Hhipl1^−/−;Apoe^−/−atherosclerotic lesions. Representative photomicrographs of atherosclerotic lesion components in Hhipl1^+/+;Apoe^−/− and Hhipl1^−/−;Apoe^−/− mice. A, Oil red O (ORO) staining for lipids; C, MOMA-2 staining for macrophages; E, anti-α smooth muscle actin (SMA) staining for smooth muscle cells; and G, Masson’s trichrome for collagen. Bars, 200 μm. Percentage coverage (average of 9 sections per animal) of (B) lipids, (D) macrophages, (F) smooth muscle cells (P=0.001), and (H) collagen (n=6–10 per group). Error bars represent mean±CI. HHIPL1 indicates hedgehog interacting protein-like 1. *P≤0.05, **P≤0.01. Bar, 200 μm.