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. Author manuscript; available in PMC: 2020 Aug 6.
Published in final edited form as: Biochemistry. 2019 Jul 26;58(31):3377–3385. doi: 10.1021/acs.biochem.9b00076

Figure 3. Structural characterization of the human 18S rRNA.

Figure 3.

(A) (top) Model of the full-length 18S rRNA with arcs representing correct, incorrect, and missing base pairs relative to the accepted structure. (middle) SHAPE reactivity (black) and entropy (brown) shown as medians over 51-nt centered windows along the full-length 18S rRNA with the axes crossing at 0.3 and 0.08, respectively. (bottom) SHAPE-directed structure models for lowSS regions modeled as independent elements. (B) Nucleotide-level models of well-structured regions of the 18S rRNA. Nucleotides are colored by SHAPE reactivity (legend in Fig. 1). Missed and incorrect base pairs are shown with red and purple arcs or lines, respectively; in general, these base pairs are supported by the experimental SHAPE reactivities, but are not consistent with the accepted structure.