KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-DIG AP	Sigma Aldrich	SKU 11093274910
Bacterial and Virus Strains
NEB 5-alpha	New England Biolabs	Cat No C2987H
SW105	[75]	N/A
Biological Samples
Chemicals, Peptides, and Recombinant Proteins
NBT	Sigma Aldrich	SKU 11383213001
BCIP	Sigma Aldrich	SKU 11383221001
Digoxigenin-labeled nucleotides	Sigma Aldrich	SKU 11277073910
Paraformaldehyde	Sigma Aldrich	SKU P6148–500G
Proteinase K	Sigma Aldrich	SKU P6556–1G
Bovine Serum Albumin	Sigma Aldrich	SKU A8022–50g
Methycellulose	Sigma Aldrich	SKU 1424506–1G
Glycerol	Sigma Aldrich	SKU G7893–1L
Tricaine (Ethyl 3-aminobenzoate methanesulfonate)	Sigma Aldrich	SKU E10521–50G
Low melt agarose (AquaPor HR)	National Diagnostics	Cat No EC-205 (100g)
BsrI	New England Biolabs	R0527S
DdeI	New England Biolabs	R0175S
Critical Commercial Assays
Deposited Data
Experimental Models: Cell Lines
Experimental Models: Organisms/Strains
cxcr4bt26035	[67]	N/A
cxcr4aum2	[68]	N/A
cldnb:lyn2GFP	[21]	N/A
prim:lyn2mCherry	[73]	N/A
cxcl12at30516	[25]	N/A
cxcr4a:H2A-mCherry	This study	N/A
cxcr4b:H2A-mCherry	[73]	N/A
cxcr4b:cxcr4a	This study	N/A
fgf10atbvbo	[72]	N/A
fgf3t24149	[71]	N/A
cdh1tx230	[34]	N/A
cdh2m117	[36]	N/A
cdh1:cdh1-sfGFP	[41]	N/A
cdh2:cdh2-mCherry	This study	N/A
cxcr4b:H2A-GFP	[30]	N/A
cxcr4b:zGrad	[41]	N/A
Gt(ctnna-citrine)Ct3a	[39]	N/A
Oligonucleotides
For primer sequences used in this study, see Table S1	Integrated DNA Technologies	Custom synthesis
Recombinant DNA
CHORI-211–75O1 (cdh2 BAC)	BACPAC Resources Center	N/A
CHORI73–268G8 (cxcr4a BAC)	BACPAC Resources Center	N/A
DKEY169F10 (cxcr4b BAC)	Source Bioscience	N/A
Software and Algorithms
Prism 8 (Version 8.1.2)	Graphpad
FIJI	N/A	https://fiji.sc/
MatLab	Mathworks	https://www.mathworks.com/
Imaris 8.0	Oxford Instruments	https://www.oxinst.com/
Python 2.7	N/A	https://www.python.org/
Data S1–S4	This study	N/A
Other