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. 2019 Jul 29;15(7):e1007938. doi: 10.1371/journal.ppat.1007938

Fig 6. Clathrin and dynamin, but not caveolin are required for the NS1-mediated disruption of the EGL.

Fig 6

(A) HPMEC were transfected with the indicated siRNA for 72 hours then treated with 5 ug/ml DENV2 NS1. The integrity of the EGL on HPMEC was assessed 6 hours post NS1-treatment by the presence of heparan sulfate (green) [9] on the cell surface. Nuclei were stained with Hoechst (blue). Images (20X; scale bars, 50 μm). (B) Quantification of MFI in (A) from 3 independent experiments. ns = not significant, **, P < 0.01.