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. 2019 Jul 29;17(7):e3000377. doi: 10.1371/journal.pbio.3000377

Fig 1. Localization of Atg11 to the PAS is influenced by Atg17.

Fig 1

(A) Schematic drawing illustrates the addressed question. Note that the proteins are not drawn to scale and that the cartoon for Atg11 represents a theoretical model since no structural information of Atg11 is available. The percentage of cells with Atg11-GFP puncta was assessed in WT and atg17Δ cells during vegetative growth and under starvation. (B) Percentage of cells with GFP-Atg11 puncta that colocalized with mCherry-Atg8 puncta in WT and atg17Δ cells during vegetative growth and under starvation. (C) Representative fluorescence images of data shown in (B). Black and white arrowheads indicate positions of Atg8 puncta, which are colocalizing (black) or not colocalizing (white) with Atg11. Scale bars 5 μm. (A and B) Data are presented as mean values ± SD of n = 3 independent experiments. Flattened z-stacks of >160 cells per condition were examined using a Fiji script. P values were calculated using a two-tailed Student t test (*P < 0.05, **P < 0.01). See also S1 Fig. GFP, green fluorescent protein; PAS, phagophore assembly site; WT, wild-type.