Fig 8. GSEA of putative paraflagellar rod proteins.
The left panel shows a heat map of enrichment in adherent (Adh) or swimming (Swim) forms for the three individual replicates (bottom of image). Gene identifiers and TriTrypDB gene name are shown. Red color indicates transcript enrichment in swimming form and blue indicates enrichment in adherent form. The 25 genes below the red dashed line (GSEA software threshold) are considered as significantly contributing to the enrichment of the set. The right panel shows the enrichment plot generated by the GSEA software with the Normalized Enrichment Score (NES) and False Discovery Rate (FDR) indicated for this gene set. A negative NES indicates enrichment in swimmers. The gray shaded area shows a rank order of the genes based on their enrichment in adherent (left, red) or swimming forms (right, blue). The black vertical lines indicate the position of the 46 members of the set within the rank order. The density of lines on the right indicates the strong enrichment of genes in swimming form. The green line indicates the enrichment profile generated by the software.