Fig 9. Differentially regulated genes in C. fasciculata-infected Ae. aegypti hindguts.

(A) PCA analysis shows that before batch correction (left) the most significant source of variation is experimental replicate (Rep; different colors). Applying batch correction (right) separates control, uninfected (open circles) from infected samples (solid circles) along PC1, which now accounts for 47% of the variation between samples. (B) Volcano plot showing changes in gene expression in infected hindguts compared to uninfected hindguts. A positive value indicates an Ae. aegypti transcript that is upregulated in infected hindguts. Red dots indicate transcripts that are significantly differentially expressed. Numbers of differentially expressed genes with a log₂ fold change of at least 0.59 and adjusted P value < 0.05 are shown in the inset.