Fig 5. CXCR3 receptor is important to cytotoxicity of specific CD8⁺ T cells during the immunization.

a-Histograms represent the frequency of CFSE^low and CFSE^high population that were transferred to βgal, ASP2, and ASP2+αCXCR3 groups. After 12 hours of transference, the percentage of CFSE^high cell lyses was calculated as described in the methods section. b-Cytotoxicity percentage of specific CD8⁺ T cells during immunization and treatment with anti-CXCR3. c-Percentage of cytotoxicity of specific CD8⁺ T cells during immunization, infection and treatment with anti-CXCR3 d-Granzyme B MFI on specific CD8⁺ T cells from βgal+T.cruzi, ASP2+T.cruzi, and ASP2+αCXCR3+T.cruzi groups. Results are shown as individual values and as the mean ± SEM for each group (n = 3). One of three independent experiments is presented. Statistical analysis was performed using the One-Way ANOVA and Tukey’s HSD tests. Symbols indicate that the values observed were significantly different between the groups (*p < .0001; **p < .01; ^#p < .0001; ^ζp = 0.0001) and n.s means no significant.