KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-Pax6 (clone 018-1330) | BD Biosciences | Cat# 561462; RRID:AB_10715442 |
| Rabbit polyclonal anti-Tbr2 | Abcam | Cat# ab23345; RRID:AB_778267 |
| Rabbit polyclonal anti-GFAP | DAKO | Cat# Z0334; RRID:AB_10013382 |
| Rat monoclonal anti-Ki-67 | DAKO | Cat# M7249; RRID:AB_2250503 |
| Rabbit polyclonal anti-phospho Histone H3 | CST | Cat# 9701; RRID:AB_331535 |
| Rabbit polyclonal anti-FMRP | CST | Cat# 4317S; RRID:AB_1903978 |
| Rabbit polyclonal anti-CRM1 | Bethyl Labs | Cat# A300-469A; RRID:AB_451004 |
| Rabbit polyclonal anti-Lamin A/C | CST | Cat# 2032; RRID:AB_2136278 |
| Mouse monoclonal anti-Beta-tubulin | Fisher | Cat# A2228; RRID:AB_476697 |
| Mouse monoclonal anti-Fascin | SCBT | Cat# sc-21743; RRID:AB_627580 |
| Rabbit polyclonal anti-PTCH1 | Proetintech | Cat# 17520-1-AP; RRID:AB_2176561 |
| Rat monoclonal anti-DLL1 | Millipore/Sigma | Cat# MABN2284 |
| Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) | Jackson Immunoresearch | Cat# 111035003 |
| Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L) | Jackson Immunoresearch | Cat# 115035003 |
| Cy3 AffiniPure Donkey Anti-Rabbit IgG (H+L) | Jackson Immunoresearch | Cat# 711165152 |
| Cy2 AffiniPure Donkey Anti-Mouse IgG (H+L) | Jackson Immunoresearch | Cat# 715225150 |
| Cy2 AffiniPure Donkey Anti-Rat IgG (H+L) | Jackson Immunoresearch | Cat# 712225153 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| FMRP Recombinant Protein (isoform 1) | AB Clonal | N/A |
| EdU (5-ethynyl-2-deoxyuridine) | Invitrogen | Cat# A10044 |
| Paraformaldehyde | Sigma | Cat# P6148-1kg CAS 30525-89-4 |
| PFA, 16% ultrapure EM grade | Polysciences | Cat# 18814-20 |
| Target Retrieval Solution | Agilent/DAKO | Cat# S1699 |
| 7-AAD | Thermo Fisher Scientific | Cat# A1310 |
| StemPro Accutase | Fisher Scientific | Cat# A1110501 |
| SUPER Rase In RNase inhibitor | Fisher Scientific/Ambion | Cat# AM2696 |
| Matrigel | Corning | Cat# #354234 |
| DMEM-F12 | Fisher Scientific/GIBCO | Cat# 11320-033 |
| N-2 Supplement | Fisher Scientific/GIBCO | Cat# 17502048 |
| B27 serum free supplement | Fisher Scientific/GIBCO | Cat# 17504-044 |
| Glutamax | Fisher Scientific/GIBCO | Cat# 35050061 |
| Recombinant Human bFGF | PeproTech | Cat# 100-18B |
| recombinant murine EGF | PeproTech | Cat# 315-09 |
| Heparin | J.T. Baker/Avantor | Cat # M916-00 |
| Complete, Mini, EDTA-free Protease Inhibitor Cocktail | Roche | Cat# 11836170001 |
| Proteinase K | VWR | Cat# 97062-670 |
| Normal Goat Serum | Jackson Immunoresearch | Cat# 005000121 |
| Normal Donkey Serum | Jackson Immunoresearch | Cat# 017000121 |
| Bovine Serum Albumin, Fraction V, Heat Shock Treated | Fisher Scientific | Cat# BP1600100 |
| Leptomycin B | SCBT | Cat# sc-358688, CAS 87081-35-4 |
| TRIzol | Invitrogen | Cat# 15-596-026 |
| Critical Commercial Assays | ||
| Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit | Invitrogen | Cat# C10635 |
| Click-iT Plus EdU Alexa Fluor 647 Imaging Kit | Invitrogen | Cat# C10640 |
| Chemiluminescent Nucleic Acid Detection Module | Fisher Scientific/Pierce | Cat# PI89880 |
| NE-PER Nuclear and Cytoplasmic Extraction Kit | Thermo Fisher Scientific | Cat# PI78835 |
| QuantaBio PerfeCTa SYBR® Green SuperMix with Low ROX | VWR | Cat# 101414-162 |
| QuantaBio qScript cDNA SuperMix | VWR | Cat# 101414-106 |
| Quant-iT RNA HS Assay Kit | Invitrogen | Cat# Q32852 |
| TruSeq RNASeq Library Prep Kit | Illumina | Cat# RS-122-2001 |
| Deposited Data | ||
| Fmr1 Nuclear Fraction RNA Seq data | This paper | GSE121809 |
| Experimental Models: Cell Lines | ||
| Nestin-Cre+/+; Mettl14f/f –derived NPCs | Yoon et al., 2017 | N/A |
| Experimental Models: Organisms/Strains | ||
| B6.129P2-Fmr1tm1Cgr/J | Jackson Laboratory | RRID:IMSR_JAX:003025 |
| Oligonucleotides | ||
| m6A Dll1 oligo: 5′-GAACACCAACAAGAAGGCGGXCUUUCACGGGG m6ACCAUGGAGCCGA-3′ | This paper | N/A |
| Non-m6A Dll1 oligo: 5′-GAACACCAACAAGAAGGCGGXCUUUCACGGGGACCAUGGAGCCGA-3′ | This paper | N/A |
| m6A short oligo: 5′-CGUGG m6ACUGGCU-3′ | This paper | N/A |
| Non-m6A short oligo: 5′-CGUGGACUGGCU-3′ | This paper | N/A |
| m6A mutated oligo: 5′-GAUAC m6AGAGAAG-3′ | This paper | N/A |
| Non-m6A mutated oligo: 5′-GAUACAGAGAAG-3′ | This paper | N/A |
| Primers for qPCR, see Table S1 | This paper | N/A |
| Software and Algorithms | ||
| FlowJo | FlowJo, LLC | RRID:SCR_008520, https://www.flowjo.com |
| TOPPGENE | Chen et al., 2009 | RRID:SCR_005726, https://toppgene.cchmc.org |
| GeneMANIA | Warde-Farley et al., 2010 | RRID:SCR_005709, http://genemania.org |
| Consensus Path-DB | Herwig et al., 2016 | http://cpdb.molgen.mpg.de |
| R | R Project for Statistical Computing | RRID:SCR_001905, https://www.r-project.org |
| MATLAB | MathWorks | RRID:SCR_001622, https://www.mathworks.com/products/matlab.html |
| Prism7 | GraphPad | SCR_002798; https://www.graphpad.com |
| Other | ||
| Protein A Dynabeads | Invitrogen | Cat# 10-001-D |
| Protein A/G PLUS-Agarose | SCBT | Cat# sc-2003; RRID:AB_10201400 |
| Kinematica Polytron PT 1200E Electric Homogenizer | Fisher Scientific | Cat# 05-400-261 |
| 5 Prime Phase Lock Gel Heavy 2 ml | VWR | Cat #10847-802 |
| BioDYNE B nylon membrane | Fisher Scientific/Pierce | Cat #77016 |
| Dip and Read Anti-Penta-HIS (HIS1K) Biosensors | Forte Bio | Cat #18-5120 |