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. Author manuscript; available in PMC: 2019 Aug 8.
Published in final edited form as: Neurogenetics. 2007 Jan 11;8(2):121–130. doi: 10.1007/s10048-006-0078-5

Fig. 4.

Fig. 4

Nf1 qPCR product formation in reactions using Nf1 primer set number 1 (a, b), Nf1 primer set number 2 (c, d), and Nf1 exon 9a primer set number 1 (e, f) is linear with respect to sequential template dilution. a, c, e Annealing temperature curves of the number of cycles required for product formation to reach threshold (Ct). b, d, f Determination of linearity regression values (R2) for the formation of product versus serial dilution