Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) | Plk1flox | Pubmed | PMID: 27417127 | Dr. Guillermo de Cárcer (Spanish National Cancer Research Centre) |
| Genetic reagent (M. musculus) | Myodcre | Jackson Laboratory | Stock #: 014140 RRID:IMSR_JAX:014140 |
|
| Genetic reagent (M. musculus) | Pax7creER | Jackson Laboratory | Stock #: 012476 RRID:IMSR_JAX:012476 |
|
| Antibody | Pax7(PAX7) mouse monoclonal | Developmental Studies Hybridoma Bank | Cat# pax7 RRID:AB_528428 | IHC (1:10) |
| Antibody | MyoG mouse monoclonal | Developmental Studies Hybridoma Bank | Cat# PCRP-MYOG-1C5 RRID:AB_2722260 | IHC (1:500) WB (1:1000) |
| Antibody | MF20 mouse monoclonal | Developmental Studies Hybridoma Bank | Cat# MF 20 RRID:AB_2147781 |
IHC (1:50) WB (1:200) |
| Antibody | eMyHC mouse monoclonal | Developmental Studies Hybridoma Bank | Cat# F1.652 RRID:AB_528358 |
IHC (1:100) |
| Antibody | Plk1 rabbit polyclonal | Cell Signaling | Cat# 4535 RRID:AB_2252687 |
IHC (1:500) WB (1:2000) |
| Antibody | MyoD mouse monoclonal | Santa Cruz Biotechnology | Cat# sc-377460 | IHC (1:300) |
| Antibody | Dystrophin rabbit polyclonal | Abcam | Cat# ab15277 RRID:AB_301813 |
IHC (1:1000) |
| Antibody | Ki67 rabbit polyclonal | Abcam | Cat# ab15580 RRID:AB_443209 |
IHC (1:1000) |
| Antibody | Phospho-Histone H3 rabbit polyclonal | Cell Signaling Technology | Cat# 9701 RRID:AB_331535 | WB (1:1000) |
| Antibody | Phospho-ATR rabbit monoclonal | Cell Signaling Technology | Cat# 30632 RRID:AB_2798992 | WB (1:1000) |
| Antibody | p53 rabbit polyclonal | Cell Signaling Technology | Cat# 9282 RRID:AB_331476 | WB (1:1000) |
| Antibody | Cleaved Caspase-3 rabbit polyclonal | Cell Signaling Technology | Cat# 9661 RRID:AB_2341188 | IHC (1:500) |
| Antibody | Anti-H2A.X, phospho mouse monoclonal | Abcam | Cat# ab26350 RRID:AB_470861 | IHC (1:1000) |
| Antibody | 488 goat polyclonal antimouse IgG1 | Thermo Fisher Scientific | Cat# A-21121 RRID:AB_141514 | IHC (1:1000) |
| Antibody | 488 goat polyclonal antirabbit IgG | Thermo Fisher Scientific | Cat# A-11034 RRID:AB_2576217 | IHC (1:1000) |
| Antibody | 568 goat polyclonal antimouse IgG1 | Thermo Fisher Scientific | Cat# A-21124 RRID:AB_2535766 | IHC (1:1000) |
| Antibody | 488 goat polyclonal antirabbit IgG | Thermo Fisher Scientific | Cat# A-21244 RRID:AB_2535812 | IHC (1:1000) |
| Antibody | HRP-conjugated goat polyclonal anti-rabbit IgG | Jackson ImmunoResearch Labs | Cat# 111-035-003 RRID:AB_2313567 | WB (1:10000) |
| Antibody | HRP-conjugated goat polyclonal anti-mouse IgG | Jackson ImmunoResearch Labs | Cat# 115-035-003 RRID:AB_10015289 | WB (1:10000) |
| Chemical compound, drug | BI2536 | Selleckchem | Cat# S1109 | |
| Chemical compound, drug | Pifithrin-α | Sigma | Cat# P4359 | |
| Chemical compound, drug | CGK733 | Sigma | Cat# C9867 | |
| Chemical compound, drug | propidium iodide | Sigma | Cat# P4170 | |
| Chemical compound, drug | Annexin V | Invitrogen | Cat# V13241 |